TECHNIQUES

TECHNIQUES: What We Do

OPTOGENETICS

VIRUS-BASED CIRCUIT RECONSTRUCTION

MICROFLUIDICS AND MICROELECTRODES

2-PHOTON AND VIDEOMICROSCOPY

LIGHT-INDUCED AXON REMODELING

MOUSE BEHAVIOR

INTRACELLULAR DYNAMICS

CALCIUM IMAGING

TECHNIQUES: What We Do

From cellular dynamics to network analyses

We use a cross-disciplinary approach based on the combination of complementary techniques:

in vitro brain-on-a-chip devices, including molecular cloning, virus production, structural analyses of axons (A) and synaptic connections (B), and functional analyses of Glutamate transmission (C) and global network activity (D).
in vivo assays in live mice, including virus-based anatomical reconstruction (E), electrophysiological recording (F), 2-photon calcium imaging (G), and motor behaviors (H).

Network analyses will be carried out in live mice using (E) viral-based reconstruction, (F) electrophysiological recording, (G) 2-photon calcium imaging, and (H) motor behaviour.

The systematic and reciprocal transition from microfluidic systems to live animals allows us to quickly respond to potential issues with optimal solutions

TECHNIQUES: Image

RESEARCH ETHICS

All experimental procedures are performed under the supervision of authorized investigators, in an authorized establishment (Institut de Neuroscience la Timone, CNRS UMR7289, authorization #B13-014-04) and in strict accordance with the Amsterdam protocol on animal protection and welfare, and the Directive 2010/63/EU of the European parliament. Our research program is fully dedicated to following the 3Rs guiding principles for animal research.

TECHNIQUES: Text